Product Details | |
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Synonyms | F-actin Cross-linking Protein |
Product Type | Polyclonal Antibody |
Properties | |
Source/Host | Rabbit |
Immunogen/Antigen | Synthetic peptide corresponding to aa 27-38 of the N-terminal α-Actinin-4, coupled via a C-terminal cysteine residue to KLH. |
Application |
Immunocytochemistry: (1:250- 1:1'600) Immunohistochemistry: (1:250- 1:1'600) Immunoprecipitation: (1:10) Western Blot: (1:1'000-1:2'500) Optimal conditions must be determined individually for each application. |
Crossreactivity |
Hamster Human Mouse Pig Rat |
Specificity | Recognizes human, mouse, rat, pig and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3. |
Purity | Antigen affinity purified. |
Concentration | 250μg/ml |
Formulation | Liquid. In PBS containing 1mg/ml BSA and 0.01% sodium azide. |
Other Product Data | Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500 μg supplied at 5mg/ml in SDS sample buffer. Use 5μl (25μg) per lane for Western blotting. |
Accession Number | UniProt ID O43707: alpha-Actinin-4 (human) |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Avoid freeze/thaw cycles. |
Use/StABIlity | Stable for at least 1 year after receipt when stored at -20°C. |
Documents | |
MSDS |
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Product Specification Sheet | |
Datasheet |
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